Coding

Part:BBa_K925002:Design

Designed by: Michael Paul Lockhart   Group: iGEM12_St_Andrews   (2012-09-24)


HisTagGST


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 292
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 292
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the design of this part our major difficulty was trying to add a very small peptide sequence into the pGEX plasmid.


Source

This part was produced by biologically adding a previously chemically synthesised peice of DNA sequence known to bind nickel on to the end of the GST protein.

References